(Top) Representative micrographs of several E. After incubation, samples were retrieved and prepared for TEM analysis, as described in Materials and Methods. The cells were induced with 0.5 mM IPTG for 16 h at 21☌ (left) or 37☌ (right) in LBG. coli BL21 (DE3) pRIL carrying the plasmid pARR10. Recombinant proteins were expressed in E. (B) Effect of the temperature on CdeC ultrastructure. The cells were induced with 0.5 mM IPTG for 16 h at 37☌. coli BL21 (DE3) pRIL carrying the plasmids pARR19 and pARR10 expressing CdeC cloned in plasmid pET22b (left) or pETM11 (right), respectively.
(A) Effect of the plasmid on the CdeC ultrastructure. Effect of plasmid backbone and temperature of expression on the multimerization and ultrastructure of CdeC.
